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One of the most important crops in the north of Iran is Tea (Camellia sinensis (L.) O. Kuntze), Today, many of these plants are at risk of disappearing therefore having information about genetics can help in designing breeding programs. In this investigation, the genetic diversity of 23 tea plants from west of Mazandaran Province, one of the important regions of producing tea in Iran, and two Iranian cultivars were studied using 21 quantitative and qualitative morphological traits and 10 ISSR markers. In morphological analyses, eigenvalues of the first eight PCs were greater than one indicating that those eight PCs significantly contributed to the variation in the genotypes studied. According to the data, the samples’ similarity ranged from 0.121 to 0.543 with an average of 0.367. In cluster analyses, samples were placed in four groups at 0.28 of coefficient dissimilarity. In ISSR analyses polymorphism percent and PIC analysis ranged between 54.55-100 and 0.35-0.49 respectively. From these results, it can be seen that these primers can detect genetic differences very well. The similarity matrix showed range of similarity was from 0.344 to 0.920 with an average of 0.640. The 25 tea genotypes were grouped into four groups by UPGMA cluster analysis based on ISSR data. In a two?dimensional plot (2D plot) generated from PCoA that was generated based on ISSR data of studied samples, four groups were obtained. From the results of this study, two points can be concluded: (1) Morphological and ISSR markers are useful instruments for the Identification of differences between tea samples, and (2) There is high genetic diversity between tea plants due to the sexual reproduction of tea plants in the past in Iran.