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Bioagrica

BIOAGRICA 2020, 1(1), 1-10


Study of Genetic Diversity between Some Tea Genotypes from Foman-Iran


Authors

Koorosh Falakro 1, Shahin Jahangirzadeh Khiavi 1, Sanam Safaei Chaeikar 1


Tea Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Lahijan, Iran
ABSTRACT

One of the most important products in the northern region of Iran especially Guilan province is the tea plant (Camellia sinensis (L.) O. Kuntze) and plays an important role in the region’s economy. Since today many tea plants in the region are being destroyed for various reasons, so having information about the genetics of those trees is helpful in designing breeding programs to reach appropriate plants for specific purposes and conservation of tea germplasm. RAPD markers, using 15 primers, were used to study the genetic relationships of 16 tea plant samples from west of Guilan province (Foman). In total, these 15 primers produced 135 scorable bands, 71.85 percent of which were polymorphic (97 bands) and 38 bands were shown monomorphic pattern (28.15 percent). The calculated PIC for all combinations was from 0.38 to 0.50 at an average of 0.49. Data analysis was performed by NTSYS software using Jaccard’s similarity coefficient to determine the amount of similarity and the dendrogram was drawn based on UPGMA. Based on molecular data, the range of similarity between samples varied from 0.484 to 0.867. Samples were divided into three groups at a similarity level of 0.44. The second group (B) was divided into two subgroups at a similarity level of 0.50. Based on released data can be concluded that there is high variability between samples of tea. In general, the study of genetic diversity showed that the RAPD marker could be useful in identifying polymorphic regions and estimating genetic distances and germplasm management in tea plants.


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