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WH 2020, 7 pages - Article ID: WH-2101022112297
The effect of peppermint essential oil on Shigella dysenteriae in Wistar rats with regard to the health of working people
Infectious Diseases Research Center, Arak University of Medical Sciences, Arak, Iran
Young Researchers and Elite Club, Arak Branch, Islamic Azad University, Arak, Iran
ABSTRACT
Introduction: Shigella dysenteriae is a gram-negative bacterium that causes bacillary dysentery or shigellosis. Dysentery, caused by Shigella dysenteriae, is a disease in poor and crowded communities and as an occupational disease can be contracted in workplaces such as agriculture, animal husbandry, tanning, hospital staff and patients. Mint with the scientific name of Mentha, in addition to medicinal use, it is used as a flavoring in the production of various food products.
Methods: In this study, after collecting and drying the plant leaves, the essential oil was extracted by water distillation (Hydro distillation) by Clevenger apparatus and the essential oil compounds were identified by GC-MS. Took place. The antimicrobial effects of essential oil were determined by MIC (minimum inhibitory concentration) & MBC (minimum bactericidal concentration). Then, 0.1 cc of the effective concentration of essential oil was injected, with insulin syringe, to the infected rats with shigellosis for 7 days.
Results: In the disk diffusion method MIC, at 0.6 % concentration of essential oil, the diameter of the growth inhibition zone was 15 mm. The minimum bactericidal concentration was observed at 0.6 % concentration of essential oil. In an animal test, after 7 days of intraperitoneal injection in rats, the infection of S. dysenteriae was almost zero.