Journal Menu
Related Events
Work Health
WH 2020, 5 pages - Article ID: WH-2101142112301
Evaluation of the effect of diluted essential oil of Zataria multiflora with nanotechnology on Pseudomonas aeruginosa in hospitals
Infectious Diseases Research Center, Arak University of Medical Sciences, Arak, Iran.
Department of Chemistry, Faculty of Science, Arak Branch, Islamic Azad University, Arak, Iran
ABSTRACT
Introduction: Pseudomonas aeruginosa is an opportunistic pathogen that has been identified as one of the most important nosocomial pathogens in recent years. This bacterium is responsible for 11 to 13% of nosocomial infections, especially in patients with cystic fibrosis, people with burns or immunodeficiency, and people undergoing artificial ventilation. Pseudomonas aeruginosa is highly resistant to many antimicrobials and antiseptics, and the presence of strains with multiple drug resistance is a major problem in the treatment of bacteria in important hospital wards such as burns and intensive care. The aim of this study was to evaluate the effect of thyme essential oil on resistant Pseudomonas isolated from patients with cystic fibrosis.
methods: First, the bacterium was isolated from the sputum sample of a cystic fibrosis patient and its identity was determined and its pattern of antibiotic resistance was determined during disk diffusion. Microplate and checkboard methods were investigated.
Results: The antibacterial effects of thyme essential oil against Pseudomonas were confirmed. In Kirby-Bayer method, up to 0.312 mg / ml halo, no growth inhibition was observed in bacteria. In the microplate dilution method, a concentration of 5 mg / ml was obtained as a result of MIC.
Conclusion: From the findings of this study, it was concluded that thyme essential oil can have significant antibacterial effects and subsequently its effects on the clinical and resistant strain of Pseudomonas aeruginosa can cause bacterial resistance failure and accelerate its treatment in the clinical ward.