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EML 2023, 5 pages - Article ID: EML-2308162112855
Isolation and Identification of Respiratory Viruses from Broiler Flocks with Respiratory Complex in west part of Mazandaran Province
a Department of Veterinary, Faculty of Agriculture, Islamic Azad University, Challous Branch, Mazandaran, Iran
b Razi Vaccine and Serum Research Institute, Karaj, Iran
c Department of Veterinary, Islamaic Azad University, Babol Branch, Mazandaran, Iran
ABSTRACT
For isolation and identification of viruses that cause respiratory complex in broiler flocks, samples were taken from 20 broiler flocks with respiratory disease in the west of Mazandaran province. Samples were taken with history recording such as general status, age of flock, and vaccination program, from the trachea, lungs, kidney, caecal tonsils, and in cases with nervous signs from the head. A 10 percent suspension of samples in BPS solution was prepared and inoculated to 10-day-old fertile SPF eggs via the allantoic route. And then hemagglutination (HA) and hemagglutination inhibition (HI) properties for Newcastle disease (ND) and subtype H9N2 of Avian Influenza (AI) viruses were considered. For identification of infection bronchitis virus, samples were passaged 3 to 4 times and the presence of the virus was confirmed by typical IBV embryo effects such as dwarfing, curling, or death. The viral RNA was extracted from the harvested allantoic fluid by using RNA purification kit (Roche Germany). A fragment of S1 segment was amplified by universal primers of IB virus in RT-PCR reaction and then Nested-PCR with specific primers for differentiation of Mass and 793/B serotype was conducted. Results revealed, that 11 samples were positive for IB virus, 3 samples were positive for ND virus and one sample was positive for AI+IB viruses’ simultaneous infection.
Respiratory Complex; broiler flock; Infectious bronchitis virus; Newcastle disease virus; Avian Influenza (H9N2) virus.